4QCE

Crystal structure of recombinant alkali thermostable GH10 xylanase from Bacillus sp. NG-27

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	54.770, 75.650, 176.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.680 - 2.320
R-factor	0.1813
Rwork	0.179
R-free	0.22690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.123
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	88.475	26.064	2.460
High resolution limit [Å]	2.320	7.370	2.320
Rmerge		0.075	0.536
Rpim		0.043	0.308
Total number of observations		5017	22434
Number of reflections	33124
<I/σ(I)>	6.6	14.1	2.4
Completeness [%]	99.7	97.9	100
Redundancy	4.6	4.3	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.1M naCl, 0.12M MgCl2, 0.05M Tris HCl pH 8.5, 15% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K