4QCC
Structure of a cube-shaped, highly porous protein cage designed by fusing symmetric oligomeric domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-03-05 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.542 |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 272.680, 272.680, 272.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 96.407 - 7.078 |
| R-factor | 0.2843 |
| Rwork | 0.283 |
| R-free | 0.31650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.183 |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.6) |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 96.407 | 7.260 | |
| High resolution limit [Å] | 7.078 | 31.660 | 7.078 |
| Rmerge | 0.073 | 0.031 | 0.712 |
| Number of reflections | 2526 | 17 | 195 |
| <I/σ(I)> | 15.62 | 41.45 | 1.67 |
| Completeness [%] | 94.7 | 44.7 | 95.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.1 M MES, pH 6.0, 0.6 M ammonium sulfate, 1% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
