4QBL

VRR_NUC domain protein

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-09-27
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9804
Spacegroup name	C 1 2 1
Unit cell lengths	213.143, 51.180, 127.267
Unit cell angles	90.00, 125.49, 90.00

Refinement procedure

Resolution	29.673 - 2.000
R-factor	0.1766
Rwork	0.175
R-free	0.20250
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.974
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.000
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	12.5 % w/v PEG 1000, 12.5% w/v PEG 3350, 12.5% v/v MPD, 0.02 M sodium L-glutamate, 0.02 M DL-alanine, 0.02 M glycine, 0.02 M DL-lysine, 0.2 M DL-serine, 0.1 M MES/imidazole pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K