4QBJ

Crystal structure of N-myristoyl transferase from Aspergillus fumigatus complexed with a synthetic inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Detector technology	IMAGE PLATE
Collection date	2003-10-30
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.542
Spacegroup name	P 21 21 21
Unit cell lengths	54.792, 77.074, 113.237
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.100
R-factor	0.17865
Rwork	0.177
R-free	0.21745
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iic
RMSD bond length	0.018
RMSD bond angle	2.081
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	2.100
Number of reflections	28569

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	1.85-2.3M ammonium sulfate, 0.1M HEPES, 20mM MnCl2, 1mM TCEP, pH 7.1-7.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K