4QB4
Crystal Structure of Staphylococcal Nuclease mutant V23L/L25V/V66L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2011-04-19 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 |
| Unit cell lengths | 47.930, 47.930, 63.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.409 - 1.600 |
| R-factor | 0.18283 |
| Rwork | 0.180 |
| R-free | 0.23631 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.317 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.409 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.040 | |
| Number of reflections | 15001 | |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 79.1 | 11.8 |
| Redundancy | 1.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.6 M Ammonium sulfate, MPD 50-60%, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K, temperature 277.0K |
