4QB1
Structure of CBM35 from Paenibacillus barcinonensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-01-14 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.400, 47.450, 103.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.510 - 2.190 |
| R-factor | 0.21209 |
| Rwork | 0.209 |
| R-free | 0.26885 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vzp 4QAW (CBM35) |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.290 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.510 | 2.300 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Rmerge | 0.068 | 0.169 |
| Number of reflections | 10371 | |
| <I/σ(I)> | 25.7 | 12.9 |
| Completeness [%] | 97.5 | 83.8 |
| Redundancy | 11.9 | 11.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 20% PEG 6000, 0.2M Calcium chloride, 0.1M MES, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
