4QAV

The structure of Beta-ketoacyl -(acyl carrier protein) synthase II (FabF) from Neisseria meningitidis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 65
Unit cell lengths	153.489, 153.489, 66.470
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.240 - 2.100
R-factor	0.1745
Rwork	0.173
R-free	0.19640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gfw
RMSD bond length	0.006
RMSD bond angle	0.853
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1688))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	33.230	33.230	2.160
High resolution limit [Å]	2.100	8.910	2.100
Number of reflections	52092
Completeness [%]	99.6	94.9	97.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296.15	20% PEG 3350, 250mM Sodium Formate, VAPOR DIFFUSION, HANGING DROP, temperature 296.15K