4QAL
Crystal structure of C117A mutant of human acidic fibroblast growth factor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-16 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97911 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 73.736, 95.323, 105.972 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.435 - 1.500 |
R-factor | 0.1612 |
Rwork | 0.160 |
R-free | 0.18970 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 1.625 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX ((Phaser MR)) |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 59288 | |
Completeness [%] | 99.3 | |
Redundancy | 14.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.7 M sodium citrate, 0.1 M imidazole, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |