4QA8

Crystal structure of LprF from Mycobacterium bovis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	273
Detector technology	CCD
Collection date	2012-07-23
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9789
Spacegroup name	P 1
Unit cell lengths	31.514, 32.381, 51.717
Unit cell angles	76.40, 80.79, 71.23

Refinement procedure

Resolution	18.563 - 1.100
R-factor	0.1651
Rwork	0.164
R-free	0.18850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.159
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	1.240	1.120
High resolution limit [Å]	1.100	1.210	1.100
Number of reflections	68768
Completeness [%]	90.4	87.2	82

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	295.15	0.1M Tris-HCl (pH 7.8), 0.2M Magnesium chloride, 28% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K