4Q9W
mTFP*: a robust and versatile host protein at 1.00 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-08 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.520, 85.170, 63.140 |
| Unit cell angles | 90.00, 100.32, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.000 |
| R-factor | 0.13175 |
| Rwork | 0.131 |
| R-free | 0.14995 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hqk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.668 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.100 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.036 | 0.335 |
| Number of reflections | 204329 | |
| <I/σ(I)> | 15.6 | 2.8 |
| Completeness [%] | 94.9 | 88.1 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 25.9% PEG3000, 100mM MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
