4Q9T
Crystal structure of Vanderwaltozyma polyspora Nup133 Beta-propeller domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-21 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.97929 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 109.172, 133.843, 136.775 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 3.000 |
R-factor | 0.2175 |
Rwork | 0.215 |
R-free | 0.26770 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.330 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.180 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.189 | 0.025 |
Number of reflections | 20381 | |
<I/σ(I)> | 14.2 | 1.2 |
Completeness [%] | 99.7 | 98.6 |
Redundancy | 14.6 | 13.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 298 | Protein (10 mM Hepes, pH 7.5, 150 mM NaCl, 10% glycerol, 5mM DTT), Reservoir (100mM Hepes pH 8.2, 10% PEG 3350, 100mM Ammonium Sulfate), Cryoprotection (30% Glycerol), VAPOR DIFFUSION, SITTING DROP, temperature 298K |