4Q9P

Crystal structure of C16T/K12V/C117V/P134V mutant of human acidic fibroblast growth factor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-14
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.020
Spacegroup name	P 41 21 2
Unit cell lengths	53.687, 53.687, 217.973
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.244 - 1.800
R-factor	0.1749
Rwork	0.173
R-free	0.21030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.031
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX ((Phaser MR))
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.800	1.800
Number of reflections	29447
Completeness [%]	95.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	1.0 M sodium citrate, 0.1 M imidazole, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K