4Q9C
IgNAR antibody domain C3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-12 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.514 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 99.148, 99.148, 52.281 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.800 |
| R-factor | 0.25975 |
| Rwork | 0.259 |
| R-free | 0.28720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4q97 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.919 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.036 | 0.304 |
| Number of reflections | 4052 | |
| <I/σ(I)> | 22.5 | 3.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 1.0 M lithium chloride, 0.1 M citric acid, 20% PEG6000, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
