4Q94

human RPRD1B CID in complex with a RPB1-CTD derived Ser2 phosphorylated peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97926
Spacegroup name	P 21 21 21
Unit cell lengths	39.286, 70.448, 108.859
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.110 - 1.850
R-factor	0.1801
Rwork	0.178
R-free	0.22120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fld
RMSD bond length	0.014
RMSD bond angle	1.476
Data reduction software	XDS
Data scaling software	Aimless (0.1.27)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.110	43.070	1.890
High resolution limit [Å]	1.850	9.060	1.850
Rmerge	0.057	0.024	1.076
Total number of observations		1468	11708
Number of reflections	26622
<I/σ(I)>	21.7	53.5	1.9
Completeness [%]	100.0	96.7	100
Redundancy	7.1	5.4	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	2.0 M ammonium sulfate, 5% isopropanol, vapor diffusion, temperature 291K