4Q8R
Crystal structure of a Phosphate Binding Protein (PBP-1) from Clostridium perfringens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.8000 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 70.780, 105.730, 146.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.564 - 1.650 |
| R-factor | 0.13474 |
| Rwork | 0.132 |
| R-free | 0.17843 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gd5 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.944 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.564 | 1.750 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 33131 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 60 mg/mL protein, 0.1 N sodium acetate, pH 4.5, 0.2 M zinc acetate, 10% PEG3000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
