4Q88
Glycosyl hydrolase family 88 from Bacteroides vulgatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-27 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.415, 73.502, 73.183 |
| Unit cell angles | 65.30, 83.66, 90.94 |
Refinement procedure
| Resolution | 26.800 - 1.730 |
| R-factor | 0.1367 |
| Rwork | 0.135 |
| R-free | 0.16420 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.616 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 26.800 | 50.000 | 1.760 |
| High resolution limit [Å] | 1.730 | 4.690 | 1.730 |
| Rmerge | 0.082 | 0.077 | 0.350 |
| Number of reflections | 96838 | ||
| <I/σ(I)> | 16.9 | 2.09 | |
| Completeness [%] | 89.9 | 91.2 | 53.4 |
| Redundancy | 1.9 | 2 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2 M lithium sulfate, 0.1 M HEPES buffer, 25% PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
