4Q7T
Crystal structure of photoswitchable fluorescent protein PSmOrange
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.363, 50.683, 52.414 |
| Unit cell angles | 94.71, 90.03, 106.59 |
Refinement procedure
| Resolution | 52.220 - 1.940 |
| R-factor | 0.203 |
| Rwork | 0.200 |
| R-free | 0.26560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h5o |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.153 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 52.221 | 52.221 | 1.980 |
| High resolution limit [Å] | 1.940 | 5.290 | 1.940 |
| Rmerge | 0.055 | 0.042 | 0.176 |
| Number of reflections | 27702 | ||
| <I/σ(I)> | 9.9 | ||
| Completeness [%] | 84.5 | 91.4 | 56.6 |
| Redundancy | 3.3 | 3.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 55% MPD, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
