4Q7O
The crystal structure of an immunity protein NMB0503 from Neisseria meningitidis MC58
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.91928 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.383, 53.532, 59.699 |
| Unit cell angles | 90.00, 98.04, 90.00 |
Refinement procedure
| Resolution | 29.556 - 1.450 |
| R-factor | 0.1508 |
| Rwork | 0.149 |
| R-free | 0.18900 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.967 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.107 | 0.384 |
| Number of reflections | 49973 | |
| <I/σ(I)> | 24.4 | 2.76 |
| Completeness [%] | 99.5 | 94.9 |
| Redundancy | 3.4 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 0.2M MgCl, 0.1M Bis-Tris:HCl, 25% (w/v) PEG3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
