4Q6Y
Crystal structure of a chemoenzymatic glycoengineered disialylated Fc (di-sFc)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Detector technology | CCD |
| Collection date | 2013-09-13 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.730, 154.220, 66.120 |
| Unit cell angles | 90.00, 110.78, 90.00 |
Refinement procedure
| Resolution | 48.230 - 3.000 |
| Rwork | 0.260 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3do3 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (refine) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.230 |
| High resolution limit [Å] | 3.000 |
| Number of reflections | 19336 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293.15 | 0.2 M magnesium chloride, 0.1 M sodium acetate pH 5, and 20% (w/v) PEG 6000, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |
