4Q6V
LpoB C-terminal domain from Salmonella enterica (Sel-Met)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-01 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 29.350, 37.930, 98.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.300 - 1.970 |
| R-factor | 0.22328 |
| Rwork | 0.220 |
| R-free | 0.29029 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.410 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER (EP) |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.300 | 2.040 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Number of reflections | 8206 | |
| Completeness [%] | 99.7 | 98.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298.15 | 0.1M NaAc pH4.6, 2M ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 298.15K |
