4Q6O
Structural analysis of the mDAP-bound form of Helicobacter pylori Csd4, a D,L-carboxypeptidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Detector technology | CCD |
Collection date | 2013-04-03 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.2823 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.931, 66.668, 143.997 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.410 |
R-factor | 0.19056 |
Rwork | 0.189 |
R-free | 0.21188 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.306 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.430 |
High resolution limit [Å] | 1.410 | 1.410 |
Number of reflections | 94978 | |
Completeness [%] | 95.7 | 91.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 296 | 0.2M calcium chloride, 0.1M HEPES-NaOH, 25%(w/v) polyethylene glycol 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 296K |