4Q6J
Crystal Structure of EAL domain Protein from Listeria monocytogenes EGD-e
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97921 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 132.771, 72.483, 77.573 |
Unit cell angles | 90.00, 125.77, 90.00 |
Refinement procedure
Resolution | 30.069 - 1.369 |
R-factor | 0.14 |
Rwork | 0.137 |
R-free | 0.16900 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.415 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: dev_1621)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.390 |
High resolution limit [Å] | 1.370 | 1.370 |
Number of reflections | 120312 | |
<I/σ(I)> | 13.1 | 1.8 |
Completeness [%] | 96.2 | 72.2 |
Redundancy | 4.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.1 M HEPES pH 7.5, 0.8 M sodium phosphate, 0.8 M postassium phosphate, VAPOR DIFFUSION, SITTING DROP, temperature 289K |