4Q62
Crystal Structure of Leucine-rich repeat- and Coiled coil-containing Protein from Legionella pneumophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97926 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.765, 97.470, 57.240 |
| Unit cell angles | 90.00, 112.54, 90.00 |
Refinement procedure
| Resolution | 35.833 - 1.898 |
| R-factor | 0.177 |
| Rwork | 0.176 |
| R-free | 0.20900 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.009 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1161)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 33636 | |
| <I/σ(I)> | 11 | 2 |
| Completeness [%] | 95.1 | 76.8 |
| Redundancy | 3.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 289 | 0.2 M Lithium Sulfate, 0.1 M CAPS:NaOH pH 10.5, 2.0 M Ammonium Sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
