4Q5Y
Crystal structure of extended-Tudor 10-11 of Drosophila melanogaster
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-10 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.99985 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 71.247, 125.441, 151.747 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| R-factor | 0.236 |
| Rwork | 0.236 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ntk |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.068 | 0.485 |
| Number of reflections | 13606 | |
| <I/σ(I)> | 21.1 | 1.9 |
| Completeness [%] | 97.2 | 79.8 |
| Redundancy | 5.1 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 60% Tacsimate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
