4Q5W
Crystal structure of extended-Tudor 9 of Drosophila melanogaster
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 1W2B |
| Synchrotron site | BSRF |
| Beamline | 1W2B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-19 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.230, 105.678, 52.603 |
| Unit cell angles | 90.00, 100.57, 90.00 |
Refinement procedure
| Resolution | 36.957 - 1.801 |
| R-factor | 0.1824 |
| Rwork | 0.180 |
| R-free | 0.22580 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.976 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.092 | 0.531 |
| Number of reflections | 36887 | |
| <I/σ(I)> | 2.6 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.2 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.04M ammonium sulphate, 0.1M HEPES, pH 7.5, 22.5% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
