4Q5P
Lysine-Ligated Yeast Iso-1 Cytochrome C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-10 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0781 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 106.020, 106.020, 149.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.520 - 1.870 |
| R-factor | 0.17386 |
| Rwork | 0.172 |
| R-free | 0.20367 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yea |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.228 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.520 | 3.190 | 1.970 |
| High resolution limit [Å] | 1.870 | 2.780 | 1.870 |
| Rmerge | 0.072 | 0.060 | 0.597 |
| Number of reflections | 35544 | ||
| <I/σ(I)> | 29.96 | 42.88 | 4.3 |
| Completeness [%] | 99.9 | 100 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.2 M ammonium phosphate, pH 8.1, 30% w/v PEG300, 0.1 M Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
