4Q59

Crystal structure of plectin 1a actin-binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.939
Spacegroup name	C 2 2 21
Unit cell lengths	41.850, 159.823, 184.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.368 - 2.300
R-factor	0.1911
Rwork	0.188
R-free	0.24530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.246
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.370	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.125	0.424
Number of reflections	28020
<I/σ(I)>	6.9	2.2
Redundancy	4.4	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	0.05 M potassium phosphate monobasic, 20% PEG8000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K