4Q57

Crystal structure of the plectin 1a actin-binding domain/N-terminal domain of calmodulin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 210
Wavelength(s)	0.933
Spacegroup name	P 21 21 21
Unit cell lengths	59.080, 65.379, 87.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.929 - 1.800
R-factor	0.1528
Rwork	0.151
R-free	0.18680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.963
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.930	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.096	0.503
Number of reflections	31749
<I/σ(I)>	17.5	3.7
Redundancy	9.4	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	0.1 M Bis-Tris, pH 6.5, 0.2 M magnesium chloride, 13% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 277K