4Q53

Crystal structure of a DUF4783 family protein (BACUNI_04292) from Bacteroides uniformis ATCC 8492 at 1.27 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-25
Detector	DECTRIS PILATUS 6M
Spacegroup name	C 1 2 1
Unit cell lengths	108.136, 37.109, 61.376
Unit cell angles	90.00, 121.66, 90.00

Refinement procedure

Resolution	28.000 - 1.270
R-factor	0.155
Rwork	0.154
R-free	0.17600
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.725
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	PHENIX (1.8.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.002	1.320
High resolution limit [Å]	1.270	1.270
Rmerge		0.385
Number of reflections	53919
<I/σ(I)>		2.6
Completeness [%]	97.0	97.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.07M Sodium Iodide, 30.00% polyethylene glycol 3350, 0.01M ferric(III) chloride, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K
1	VAPOR DIFFUSION, SITTING DROP		277	0.07M Sodium Iodide, 30.00% polyethylene glycol 3350, 0.01M ferric(III) chloride, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K