4Q4Z

Thermus thermophilus RNA polymerase de novo transcription initiation complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-05
Detector	ADSC QUANTUM 270
Wavelength(s)	0.918
Spacegroup name	C 1 2 1
Unit cell lengths	184.487, 102.159, 294.719
Unit cell angles	90.00, 98.96, 90.00

Refinement procedure

Resolution	29.781 - 2.900
R-factor	0.2559
Rwork	0.256
R-free	0.27470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.930
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.850
Number of reflections	111819
Completeness [%]	88.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.7	300	crystallization solution [100 mM Tris-HCl, 200 mM KCl, 50 mM MgCl2, 10 mM Spermine tetra HCl and 10% PEG 4000], pH 8.7, VAPOR DIFFUSION, HANGING DROP, temperature 300K