4Q4W

High-resolution crystal structure of Coxsackievirus A24v

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976300
Spacegroup name	I 2 2 2
Unit cell lengths	306.556, 366.509, 368.101
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.890 - 1.400
R-factor	0.14994
Rwork	0.150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.129
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PYMOL
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	29.891
High resolution limit [Å]	1.400
Number of reflections	3666980
Completeness [%]	92.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	200 mM Magnesium chloride, 3.4 M 1,6-Hexanediol, 100 mM HEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K