4Q4I
Crystal structure of E.coli aminopeptidase N in complex with amastatin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 119.386, 119.386, 170.129 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.720 - 2.310 |
R-factor | 0.14178 |
Rwork | 0.140 |
R-free | 0.17888 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.018 |
RMSD bond angle | 1.826 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.750 | 2.390 |
High resolution limit [Å] | 2.310 | 2.310 |
Number of reflections | 62174 | |
Completeness [%] | 99.7 | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1.8M Sodium Malonate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |