4Q4G
Structure of the Resuscitation Promoting Factor Interacting protein RipA mutated at C383
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9343 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.757, 65.505, 67.978 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.540 - 0.970 |
| R-factor | 0.12627 |
| Rwork | 0.126 |
| R-free | 0.17462 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.174 |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.000 |
| High resolution limit [Å] | 0.970 | 0.970 |
| Number of reflections | 97211 | |
| Completeness [%] | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 5 and 8 mg mL-1 protein concentration, respectively, and 8% (v/v) 2-Propanol, 16% (w/v) PEG4000 in 60 mM Sodium citrate trihydrate buffer, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
