4Q47
Structure of the DrRecQ Catalytic Core in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2012-10-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.756, 95.610, 183.832 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.560 - 2.899 |
| R-factor | 0.2265 |
| Rwork | 0.222 |
| R-free | 0.29530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oyw |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.787 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.899 | 2.900 |
| Rmerge | 0.678 | |
| Number of reflections | 33811 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 277 | 0.1M midazole (pH7.2), 16% PEG 20K, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
