4Q45
DNA Polymerase- damaged DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-18 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.500, 57.900, 111.040 |
| Unit cell angles | 90.00, 90.44, 90.00 |
Refinement procedure
| Resolution | 51.339 - 2.176 |
| R-factor | 0.2141 |
| Rwork | 0.212 |
| R-free | 0.25670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.051 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.340 | 2.290 |
| High resolution limit [Å] | 2.176 | 2.180 |
| Rmerge | 0.058 | 0.328 |
| Number of reflections | 100631 | |
| <I/σ(I)> | 8.9 | 3.9 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 298 | 0.1M ACETATE, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
