4Q38
The crystal structure of acyltransferase in complex with decanoyl-CoA and teicoplanin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A |
| Synchrotron site | NSRRC |
| Beamline | BL15A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-21 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 65 |
| Unit cell lengths | 133.806, 133.806, 49.301 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.500 - 1.990 |
| R-factor | 0.19316 |
| Rwork | 0.192 |
| R-free | 0.22033 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mfj |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.181 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 1.990 | 2.000 |
| Number of reflections | 34606 | |
| Completeness [%] | 99.8 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1mM MES, 0.2M ammonium sulphate, 30%(V/V) PEG 5000 MME, 1mM decanoyl-CoA, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
