4Q2Y

Crystal structure of Arginyl-tRNA synthetase

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Detector technology	PIXEL
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	77.623, 105.234, 172.259
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.901 - 2.799
R-factor	0.2112
Rwork	0.208
R-free	0.26780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bs2
RMSD bond length	0.010
RMSD bond angle	1.384
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.850
High resolution limit [Å]	2.799	7.590	2.800
Rmerge	0.086	0.046	0.314
Number of reflections	33971
<I/σ(I)>	12.5
Completeness [%]	95.5	92.2	89.1
Redundancy	5.7	8	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	293	0.085mM Sodium citrate tribasic dehydrate, 24% Polyethylene glycol 4000, 0.17M Ammonium acetate, 15% Glycerol, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K