4Q2A
WNK1: A chloride sensor via autophosphorylation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.91997 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.005, 58.304, 65.043 |
| Unit cell angles | 90.00, 91.35, 90.00 |
Refinement procedure
| Resolution | 43.409 - 3.500 |
| R-factor | 0.24827 |
| Rwork | 0.243 |
| R-free | 0.29743 |
| Structure solution method | SAD |
| Starting model (for MR) | 3FPQ |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.753 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.409 | 3.560 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.288 | 0.517 |
| Number of reflections | 3290 | |
| <I/σ(I)> | 44.3 | 4.3 |
| Completeness [%] | 99.4 | 96.1 |
| Redundancy | 6.9 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 26% PEG2000 MME, 300 mM sodium bromide, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
