4Q27

Galectin-1 in Complex with a Click-Activated N-Acetyllactosamine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M-F
Spacegroup name	P 21 21 21
Unit cell lengths	43.468, 58.130, 110.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.465 - 1.200
R-factor	0.1518
Rwork	0.151
R-free	0.17970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.022
RMSD bond angle	2.053
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.465
High resolution limit [Å]	1.051	1.200
Number of reflections	102105
<I/σ(I)>	14.2
Completeness [%]	96.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K