4Q20
Crystal structure of a C-terminal part of tyrosine kinase (DivL) from Caulobacter crescentus CB15 at 2.50 A resolution (PSI Community Target, Shapiro)
Replaces: 4EW8Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-04-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 69.530, 69.530, 194.420 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.112 - 2.500 |
| R-factor | 0.2032 |
| Rwork | 0.202 |
| R-free | 0.23160 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | XSCALE (December 6, 2010) |
| Phasing software | SHELX |
| Refinement software | BUSTER-TNT (2.10.0) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.112 | 44.110 | 2.600 |
| High resolution limit [Å] | 2.500 | 5.570 | 2.500 |
| Rmerge | 0.031 | 0.011 | 0.011 |
| Number of reflections | 19457 | 1884 | 2102 |
| <I/σ(I)> | 27.12 | 63.6 | 2.1 |
| Completeness [%] | 99.1 | 97.7 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 18% PEG 10K, 0.1 M HEPES pH 7.5, MG and ADP, both at 5 mM concentrations, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K, VAPOR DIFFUSION, SITTING DROP |
| 2 |
