4Q1U
Serum paraoxonase-1 by directed evolution with the K192Q mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 93.514, 93.514, 144.007 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.470 - 2.302 |
| R-factor | 0.17773 |
| Rwork | 0.176 |
| R-free | 0.21191 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sre |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.175 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0111) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.472 | 2.340 |
| High resolution limit [Å] | 2.302 | 2.302 |
| Rmerge | 0.110 | 0.706 |
| Number of reflections | 29105 | |
| <I/σ(I)> | 27.7 | 4.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.1 | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 20% PEG3350, 0.2 M sodium bromide, 0.1 M Bis-tris propane, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
