4Q1Q
Crystal structure of TibC-catalyzed hyper-glycosylated TibA55-350 fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2012-01-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.807, 62.166, 97.288 |
| Unit cell angles | 90.00, 90.90, 90.00 |
Refinement procedure
| Resolution | 19.870 - 2.110 |
| R-factor | 0.2076 |
| Rwork | 0.205 |
| R-free | 0.24760 |
| Starting model (for MR) | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.864 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.100 |
| Number of reflections | 31569 |
| Completeness [%] | 99.4 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | 20%(w/v) PEG 8000, 100mM magnesium acetate, 100mM Tris-HCl, pH 8.0, VAPOR DIFFUSION, temperature 293K |
