4Q1L
Crystal structure of Leucurolysin-a complexed with an endogenous tripeptide (QSW).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.425 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.350, 58.408, 76.842 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.099 - 1.900 |
| R-factor | 0.1554 |
| Rwork | 0.153 |
| R-free | 0.19170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gbo |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.235 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (Crystallographic Software 2.5.3) |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
| Number of reflections | 16352 | ||
| Completeness [%] | 100.0 | 99.7 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 20% PEG 6000, 0.1 M MIB/MIB 0.1 M AMMONIUM ACETATE, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |
