4Q0R
The catalytic core of Rad2 (complex I)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9799 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 94.085, 94.085, 155.264 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.406 - 2.750 |
| R-factor | 0.2485 |
| Rwork | 0.245 |
| R-free | 0.31020 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.163 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.800 |
| High resolution limit [Å] | 2.800 | 2.750 |
| Rmerge | 0.114 | 0.466 |
| Number of reflections | 39459 | |
| <I/σ(I)> | 2.2 | |
| Completeness [%] | 98.7 | 86.5 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20% (v/v) ethylene glycol, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
