4Q0F

Crystal Structure of Thermotoga maritima FtsH Periplasmic domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-28
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97924, 0.97901, 0.97134, 0.98696
Spacegroup name	P 21 21 2
Unit cell lengths	42.032, 66.125, 72.033
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.304 - 1.948
R-factor	0.2112
Rwork	0.209
R-free	0.25430
Structure solution method	MAD
RMSD bond length	0.003
RMSD bond angle	0.639
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.980
High resolution limit [Å]	1.948	1.950
Number of reflections	15191
Completeness [%]	99.8	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	294	15% (w/v) PEG 2000, 0.1M MES-NaOH (pH 6.0) , VAPOR DIFFUSION, HANGING DROP, temperature 294K