4PZQ

Crystal Structure of CCG DNA repeats

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-09-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.56418, 1.60467, 1.60553
Spacegroup name	P 43 21 2
Unit cell lengths	38.145, 38.145, 54.249
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.240
R-factor	0.235
Rwork	0.235
R-free	0.25700
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.073
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.320
High resolution limit [Å]	2.240	2.240
Rmerge	0.040	0.274
Number of reflections	2159
<I/σ(I)>		5.851
Completeness [%]	99.2	94.4
Redundancy		8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277.15	50MM SODIUM CACODYLATE, 1MM MAGNESIUM CHLORIDE, 5% MPD, 2MM COBALT(II) CHLORIDE, 1MM CHROMOMYCIN A3, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K