4PZN
Crystal structure of PHC3 SAM L971E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-08 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.099, 60.746, 61.431 |
| Unit cell angles | 69.43, 75.88, 78.06 |
Refinement procedure
| Resolution | 19.683 - 2.300 |
| R-factor | 0.2095 |
| Rwork | 0.205 |
| R-free | 0.24950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kw4 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.237 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.680 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 19834 | |
| <I/σ(I)> | 11.4 | 3.1 |
| Completeness [%] | 97.8 | 97.2 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 55% ethylene glycol, 100 mM tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
