4PZK
Crystal strucrure of putative RNA methyltransferase from Bacillus anthracis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97916 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.249, 59.758, 111.455 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.551 - 1.500 |
| R-factor | 0.147 |
| Rwork | 0.145 |
| R-free | 0.18500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3l8u |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.103 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: dev_1593)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.600 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 51749 | |
| <I/σ(I)> | 11.5 | 2.2 |
| Completeness [%] | 98.6 | 84.9 |
| Redundancy | 7 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2M MgCl2; 0.1M Tris pH 8.5, 16% PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
