4PZ6
PCE1 guanylyltransferase bound to SER2/SER5 phosphorylated RNA pol II CTD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.406, 78.614, 115.716 |
| Unit cell angles | 90.00, 91.42, 90.00 |
Refinement procedure
| Resolution | 36.274 - 2.406 |
| R-factor | 0.1826 |
| Rwork | 0.180 |
| R-free | 0.23270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p16 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.692 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.406 | 2.406 |
| Rmerge | 0.092 | 0.410 |
| Number of reflections | 38342 | |
| <I/σ(I)> | 9.6 | 2.9 |
| Completeness [%] | 97.3 | 89.4 |
| Redundancy | 3.9 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 23% PEG400, 0.1 M sodium acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
