4PZ5
Crystal structure of the second and third fibronectin F1 modules in complex with a fragment of BBK32 from Borrelia burgdorferi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0039 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 86.580, 86.580, 63.570 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.490 - 1.960 |
| R-factor | 0.163 |
| Rwork | 0.161 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cg7 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.969 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.490 | 2.030 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.104 | 0.590 |
| Number of reflections | 10524 | |
| <I/σ(I)> | 12.9 | 4.3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.2 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 291 | 1.4 M Na/K phosphate, pH 6.9, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
